Long-Term Efficacy Evaluation of Controlled Release Systems Based on Fullerene Nanoparticles in Chronic Lung Diseases
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Abstract
This study developed a polymer-coated fullerene nanoparticle system (C₆₀@PCL) for long-term gene delivery in chronic lung inflammation. The carrier was prepared by solvent evaporation, forming uniform particles with a mean diameter of 145 nm and a narrow size distribution (PDI = 0.17). The system successfully encapsulated IL-10 plasmid DNA and showed a controlled release profile lasting over 21 days. In mouse experiments, nasal delivery of C₆₀@PCL-IL-10 maintained IL-10 expression for at least 21 days and reduced lung inflammation scores by 68% compared with the control group. Blood biochemical tests showed no abnormal values, confirming good safety. These findings indicate that the fullerene-polymer hybrid structure can provide sustained gene expression and reduce inflammation in chronic lung disease. The approach offers a simple, stable, and biocompatible platform for future pulmonary gene therapy applications.
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